Yeast surface display platform for rapid discovery of conformationally selective nanobodies
نویسندگان
چکیده
منابع مشابه
Escherichia coli surface display for the selection of nanobodies
Nanobodies (Nbs) are the smallest functional antibody fragments known in nature and have multiple applications in biomedicine or environmental monitoring. Nbs are derived from the variable segment of camelid heavy chain-only antibodies, known as VHH. For selection, libraries of VHH gene segments from naïve, immunized animals or of synthetic origin have been traditionally cloned in E. coli phage...
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Camelid single-domain antibody fragments (“nanobodies”) provide the remarkable specificity of antibodies within a single immunoglobulin VHH domain. This unique feature enables applications ranging from their use as biochemical tools to therapeutic agents. Virtually all nanobodies reported to date have been obtained by animal immunization, a bottleneck restricting many applications of this techn...
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ژورنال
عنوان ژورنال: Nature Structural & Molecular Biology
سال: 2018
ISSN: 1545-9993,1545-9985
DOI: 10.1038/s41594-018-0028-6